論文等

5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae.,Hoshida H, Kondo M, Kobayashi T, Yarimizu T, Akada R, Appl. Mcrobiol. Biotechnol. 2016; DOI: 10.1007/s00253-016-7891-z, (in press),

Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations., Matsushita K, Azuma Y, Kosaka T, Yakushi T, Hoshida H, Akada R, Yamada M., Biosci Biotechnol Biochem. 2016; 80: 655-668. doi: 10.1080/09168451.2015.1104235


Selection of Error-Less Synthetic Genes in Yeast., Hoshida H, Yarimizu T, Akada R, Methods Mol Biol. 2017; 1472: 237-246. doi: 10.1007/978-1-4939-6343-0_18, (in press)

The thermotolerant yeast Kluyveromyces marxianus is a useful organism for structural and biochemical studies of autophagy., Yamamoto H, Shima T, Yamaguchi M, Mochizuki Y, Hoshida H, Kakuta S, Kondo-Kakuta C, Noda NN, Inagaki F, Itoh T, Akada R, Ohsumi Y., J Biol Chem. 2015; 290: 29506-29518. doi: 10.1074/jbc.M115.684233

End joining-mediated gene expression in mammalian cells using PCR-amplified DNA constructs that contain terminator in front of promoter., Nakamura M, Suzuki A, Akada J, Tomiyoshi K, Hoshida H, Akada R., Mol Biotechnol. 2015; 57: 1018-1029. doi: 10.1007/s12033-015-9890-1

Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus., Suzuki A, Fujii H, Hoshida H, Akada R., FEMS Yeast Res. 2015; 15. pii: fov059. doi: 10.1093/femsyr/fov059

A novel terminator primer and enhancer reagents for direct expression of PCR-amplified genes in mammalian cells., Nakamura M, Suzuki A, Akada J, Yarimizu T, Iwakiri R, Hoshida H, Akada R., Mol Biotechnol. 2015; 57: 767-780. doi: 10.1007/s12033-015-9870-5

Mutant screening for oncogenes of Ewing's sarcoma using yeast., Kitagawa T, Okita H, Baron B, Tokuda K, Nakamura M, Wang Y, Akada J, Hoshida H, Akada R, Kuramitsu Y, Nakamura K., Appl Microbiol Biotechnol. 2015; 99: 6737-6744. doi: 10.1007/s00253-015-6621-2

Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus., Yarimizu T, Nakamura M, Hoshida H, Akada R., Microb Cell Fact. 2015; 14: 20. doi: 10.1186/s12934-015-0203-y

Screening of accurate clones for gene synthesis in yeast., Yarimizu T, Nakamura M, Hoshida H, Akada R., J Biosci Bioeng. 2015; 119: 251-259. doi: 10.1016/j.jbiosc.2014.08.006

耐熱性微生物の耐熱性機構を付与する分子機構,山田守,赤田倫治,高坂智之,東慶直,星田尚司,松下一信,化学と生物 2015; 53: 763-773

Minimum GC-rich sequences for overlap extension PCR and primer annealing, Nakamura M, Suzuki A, Hoshida H, Akada R., Methods Mol Biol. 2014; 1116: 165-181. doi: 10.1007/978-1-62703-764-8_12

Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus., Hoshida H, Murakami N, Suzuki A, Tamura R, Asakawa J, Abdel-Banat BM, Nonklang S, Nakamura M, Akada R., Yeast. 2014; 31: 29-46. doi: 10.1002/yea.2993

Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae., Yarimizu T, Nonklang S, Nakamura J, Tokuda S, Nakagawa T, Lorreungsil S, Sutthikhumpha S, Pukahuta C, Kitagawa T, Nakamura M, Cha-Aim K, Limtong S, Hoshida H, Akada R., Yeast. 2013; 30: 485-500. doi: 10.1002/yea.2985

Novel small-molecule compounds that affect cellular morphogenesis in yeast and mammalian cells., Fukunaga T, Nakamura M, Kitagawa T, Watanapokasin R, Hoshida H, Akada R., Biosci Biotechnol Biochem. 2013; 77: 1669-1676

Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae., Fukunaga T, Cha-Aim K, Hirakawa Y, Sakai R, Kitagawa T, Nakamura M, Nonklang S, Hoshida H, Akada R., Yeast. 2013; 30: 243-253. doi: 10.1002/yea.2957

N-Glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae., Hoshida H, Fujita T, Cha-aim K, Akada R., Appl Microbiol Biotechnol. 2013; 97: 5473-82. doi: 10.1007/s00253-012-4582-2

Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7., Yamaguchi M, Matoba K, Sawada R, Fujioka Y, Nakatogawa H, Yamamoto H, Kobashigawa Y,Hoshida H, Akada R, Ohsumi Y, Noda NN, Inagaki F., Nat Struct Mol Biol. 2012; 19: 1250-6. doi: 10.1038/nsmb.2451

Construction of a URA3 deletion strain from the allotetraploid bottom-fermenting yeast Saccharomyces pastorianus., Murakami N, Miyoshi S, Yokoyama R, Hoshida H, Akada R, Ogata T., Yeast. 2012; 29: 155-165. doi: 10.1002/yea.2897

Selection and identification of thermotolerant ethanol producing yeast strains, Phong T. T., Hung L. T. , Bich L. T., Phuong, Hanh VT, Hoshida H, R. Akada, Vietnam J. Biol. 2012; 34:125-131

Takao Kitagawa, Yuko Hashizume, Tatsuhiko Murakane, Emi Koga, Yuki Nomura, Yoshito Kakihara, Ayako Fujieda, Motoyuki Uchida, Hidetoshi Takahashi, Hisashi Hoshida, and Rinji Akada, Screening of Drugs that Suppress Ste11 MAPKKK Activation in Yeast Identified a c-Abl Tyrosine Kinase Inhibitor. Biosci. Biotechnol. Biochem., 97, 772-782, 2007.

Takao Kitagawa, Hisashi Hoshida and Rinji Akada, Genome-Wide Analysis of Cellular Response to a Bacterial Genotoxin CdtB in Yeast. Infect. Immun., 75, 1393-1402, 2007

赤田倫治
清酒酵母の分子育種 
発酵・醸造食品の最新技術と機能性 監修:北本勝ひこ pp. 47-57シーエムシー出版2006/7/30

Yoshito Kakihara, Yasunobu Matsuura, Hisashi Hoshida, Yoshinori Nishizawa, and Rinji Akada.
Cost-saving design of PCR primers containing additional sequences. ITE letters 6 (2) , 135-139. (2005)

Hisashi Hoshida, Takayuki Ohira, Akira Minematsu, Rinji Akada, and Yoshinori Nishizawa.
Accumulation of eicosapentaenoic acid in Nannochloropsis sp in response to elevated CO2 concentrations. Journal of Applied Phycology 2005, 17, 29-34

赤田倫治 巻頭随想 「純迷酒」 日本醸造協会誌 2003年12月

新規交配育種法による「新やまぐち・桜酵母」の取得および純米酒「春色」の開発
有富和生,橋本信嗣,椎木幹夫,永山貴博,赤田倫治,柏木亨
山口県産業技術センター研究報告 No. 16 p4-6 2004

Hisashi HOSHIDA,* Tsuneyasu FUJITA, Kenji MURATA, Kanako KUBO, and Rinji AKADA
Copper-dependent production of a Pycnoporus coccineus extracellular laccase
in Aspergillus oryzae and Saccharomyces cerevisiae.
Biosci. Biotechnol. Biochem. 69, 1090-1097, 2005

Shinji Hashimoto, Kazuo Aritomi, Takafumi Minohara, Yoshinori Nishizawa, Hisashi Hoshida, Susumu Kashiwagi, Rinji Akada: Direct mating between diploid sake strains of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 69, 689-696, 2006

赤田倫治,北川孝雄,有富和生 (解説)
実用酵母の新しい遺伝学 ―おいしいお酒への新たな道筋―
Current development of industrial yeast genetics -A new road to tasty sake-
バイオサイエンスとバイオインダストリー 63(8), 27-30, 2005

Rinji Akada, Takao Kitagawa, Shohei Kaneko, Daiso Toyonaga, Sachiko Ito, Yoshito Kakihara, Hisashi Hoshida, Shigeru Morimura, Akihiko Kondo, and Kenji Kida. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. Yeast 23, 399-405 2006

星田尚司,北川孝雄,赤田倫治
出芽酵母の超簡単形質転換法によるポストゲノム解析
和光純薬時報 73,(4) 2-4,2005Oct. ISSN 1347-4804

赤田倫治
酵母組換え体による高品質タンパク質の大量生産
中国地域バイオシーズガイドブック 2006年2月 財団法人 中国技術振興センター pp. 29-30

Shinji Hashimoto, Mayumi Ogura, Kazuo Aritomi, Hisashi Hoshida, Yoshinori Nishizawa, Rinji Akada: Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis. Appl. Environ. Microbiol., 71, 312-319 (2005)

Isao Hirosawa, Kazuo Aritomi, Hisashi Hoshida, Susumu Kashiwagi, Yoshinori Nishizawa, and Rinji Akada: Construction of a self-cloning sake yeast that overexpresses alcohol acetyltransferase gene by a two-step gene replacement protocol. Appl. Microbiol. Biotechnol. 65:68-73 (2004)

Kazuo Aritomi, Isao Hirosawa, Hisashi Hoshida, Mikio Shiigi, Yoshinori Nishizawa, Susumu Kashiwagi, and Rinji Akada: Self-cloning yeast strains containing novel FAS2 mutations produce a higher amount of ethyl caproate in Japanese sake. Biosci. Biotech. Biochem., 68, 206-214 (2004).

Rinji Akada: Genetically Modified Industrial Yeast Ready for Application. J. Biosci. Bioeng., 94, 536-544, 2002.

Rinji Akada, Isao Hirosawa, Miho Kawahata, Hisashi Hoshida and Yoshinori Nishizawa: Sets of integrating plasmids and gene disruption cassettes containing improved counter-selection markers designed for repeated use in budding yeast. Yeast 19, 393-402, 2002.

Hisashi Hoshida, Mitsuhide Nakao, Hidenobu Kanazawa, Kanako Kubo, Thoru Hakukawa, Koji Morimasa, Rinji Akada and Yoshinori Nishizawa: Isolation of Five Laccase gene sequences from the white-rot fungus Trametes sanguinea by PCR, and cloning, characterization and expression of the laccase cDNA in yeasts. J. Biosci. Bioeng., 92, 372-380, 2001.

Rinji Akada, Yoshirou Shimizu, Yuji Matsushita, Miho Kawahata, Hisashi Hoshida and Yoshinori Nishizawa: Use of a YAP1 overexpression cassette conferring specific resistance to cerulenin and cycloheximide as an efficient selectable marker in the yeast Saccharomyces cerevisiae. Yeast 19, 17-28, 2002.

Rinji Akada, Isao Hirosawa, Hisashi Hoshida, and Yoshinori Nishizawa: Detection of a point mutation in FAS2 gene of sake yeast strains by allele-specific PCR amplification. J. Biosci. Bioeng., 92, 189-192, 2001.

Rinji Akada, Miho Kawahata, and Yoshinori Nishizawa: Elevated temperature greatly improves transformation of fresh and frozen competent cells in yeast. BioTechniques 28: 854-856 (2000)

Rinji Akada, Tatsuhiko Murakane, and Yoshinori Nishizawa: DNA extraction method for screening yeast clones by PCR. BioTechniques 28: 668-674 (2000)

Rinji Akada, Kazuyoshi Matsuo, Kazuo Aritomi, and Yoshinori Nishizawa: Construction of recombinant sake yeast containing a dominant FAS2 mutation without extraneous sequences by a two-step replacement protocol. J. Bioscience and Bioengineering 87: 43-48, 1999.

Miho Kawahata, Shinji Amari, Yoshinori Nishizawa, and Rinji Akada: A positive selection for plasmid loss in Saccharomyces cerevisiae using galactose-inducible growth inhibitory sequences. Yeast 15, 1-10, 1999.

赤田倫治:実用酵母の分子育種:清酒酵母の研究-90年代の研究- (清酒酵母・麹研究会)pp. 55-62平成15年

赤田倫治:食品微生物の組換え審査事情 バイオサイエンスとバイオインダストリー 61, 52-55, 2003

赤田倫治:遺伝子組換え酵母の実用化?ゲノムを利用する酵母産業への展開?. 日本イースト工業会技術報告 第72号 平成14年 48-57

小渕茂寿,岡本栄子,赤田倫治,熊田誠,轟木雄介:1B族遷移金属板表面の抗菌性の解析. 材料と環境 第52巻 第8号 428-435(2003)

赤田倫治: PGKp-YAP1を利用した出芽酵母の形質転換. Toyobo Upload, 67, 13-14, 2002

赤田倫治,有富和生,西澤義矩: 実用化を待つ遺伝子組換え酵母. 化学と生物 38, 30-36,2000

赤田倫治: 遺伝子組換え酵母の実用化へむけて-不安を取り除く組換え新技術- 日本醸造協会誌 Vol. 93, No. 2 pp. 113-119, 1998.


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